By Paolo Di Nardo, Sanjiv Dhingra, Dinender K. Singla
This quantity features a number of protocols from many of the significant laboratories fascinated with stem mobilephone study internationally. The study mentioned during this booklet covers themes equivalent to: separating, characterizing and increasing dental pulp stem cells; manipulating the proliferative capability of cardiomyocytes by means of gene move; isolation of stromal stem cells from adipose tissue; noninvasive overview of telephone fats and biology in transplanted mesenchymal stem cells; and cell-free remedy for organ fix. Written within the hugely winning Methods in Molecular Biology series layout, chapters comprise introductions to their respective subject matters, lists of the required fabrics and reagents, step by step, quite simply reproducible laboratory protocols, and tips about troubleshooting and averting identified pitfalls.
Cutting-edge and thorough, Adult Stem Cells: tools and Protocols is a beneficial source for supporting researchers remodel the research of stem cells into an industrialized technique that might offer sufferers with effective, secure, and competitively priced mobilephone treatments.
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Additional info for Adult Stem Cells: Methods and Protocols
Zacchigna S, Giacca M (2014) Extra- and intracellular factors regulating cardiomyocyte proliferation in postnatal life. Eulalio A, Mano M, Dal FM, Zentilin L, Sinagra G, Zacchigna S, Giacca M (2012) Functional screening identifies miRNAs inducing cardiac regeneration. Liu N, Olson EN (2010) MicroRNA regulatory networks in cardiovascular development. Porrello ER (2013) microRNAs in cardiac development and regeneration. Clin Sci (Lond) 125:151–166 10. Porrello ER, Johnson BA, Aurora AB, Simpson E, Nam YJ, Matkovich SJ, Dorn GW 2nd, van Rooij E, Olson EN (2011) MiR-15 family regulates postnatal mitotic arrest of cardiomyocytes.
MSCs growth medium: one bottle of MesenCult™ MSc Basal Medium, one bottle MesenCult™ Mesenchymal Stem Cell Stimulatory Supplements, 5 mL antibiotic-antimycotic, 5 mL L-Glutamine. 25 gm collagenase I, 80 mL PBS, 20 mL fetal bovine serum (FBS) then filter sterilize the solution. 10. Parafilm. 11. 60 and 100 mm cell culture dish. 12. 5 mL round-bottom polystyrene tube. 13. 3% acetic acid in methylene blue. 14. Glass cover slip 22 × 30 mm. 15. Hemocytometer. EasySep™, Mouse Mesenchymal Progenitor Enrichment Kit (STEMCELL).
Molina ER, Smith BT, Shah SR, Shin H, Mikos AG (2015) Immunomodulatory properties of stem cells and bioactive molecules for tissue engineering. J Control Release. Gebler A, Zabel O, Seliger B (2012) The immunomodulatory capacity of mesenchymal stem cells. Trends Mol Med 18(2):128–134 13. Aoki CA, Borchers AT, Ridgway WM, Keen CL, Ansari AA, Gershwin ME (2005) NOD mice and autoimmunity. Autoimmun Rev 4(6):373–379 14. Jonsson MV, Delaleu N, Brokstad KA, Berggreen E, Skarstein K (2006) Impaired salivary gland function in NOD mice: association with changes in cytokine profile but not with histopathologic changes in the salivary gland.
Categories: Molecular Biology