By John N. Abelson, Melvin I. Simon, M. Ian Phillips
Antisense know-how is the facility to control gene expression inside mammalian cells supplying strong experimental ways for the examine of gene functionality and gene legislation. for instance, equipment which inhibit gene expression let experiences probing the conventional functionality of a particular product inside of a mobilephone. Such method can be utilized in lots of disciplines akin to pharmacology, oncology, genetics, telephone biology, developmental biology, molecular biology, biochemistry, and neurosciences. This quantity might be a really very important device in biomedically-oriented research.The severely acclaimed laboratory typical for greater than 40 years, tools in Enzymology is likely one of the such a lot hugely revered courses within the box of biochemistry. considering 1955, each one quantity has been eagerly awaited, often consulted, and praised by way of researchers and reviewers alike. Now with greater than three hundred volumes (all of them nonetheless in print), the sequence includes a lot fabric nonetheless proper today-truly an important book for researchers in all fields of existence sciences.
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Extra info for Antisense Technology, Part B
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Garzo´n, Y. Martı´nez-Pen˜a, and P. Sa´nchez-Bla´zquez, Eur. J. Neurosci. 9, 1194 (1997). f C. Kleuss, J. Hescheler, C. Ewel, W. Rosenthal, G. Schultz, and B. Wittig, Nature (London) 353, 43 (1991). g J. Garzo´n, M. Castro, and P. Sa´nchez-Bla´zquez, Analgesia 1, 4 (1995). b In Vivo Administration of Oligodeoxynucleotides ODN solutions are made up in the appropriate volume of saline immediately prior to use. Various control groups of mice are used to monitor the speciﬁcity of ODN treatments. Typically, these controls include noninjected animals (naive), those that receive the vehicle used for the ODNs (saline), and animals injected with a random sequence ODN (ODN-RD) or mismatched antisense sequence.